BerkiGEM2007-HannahConstructionFile1

From 2007.igem.org

(Difference between revisions)

Revision as of 21:30, 28 June 2007

PCR HNX-F and HNX-R on H-Nox (567bp, BglII/XhoI) Sub into pBca1144 ( BglII/XhoI, 2054+910 L) Product is pBca1144-H-NOX


HNX-F Forward primer anneals to BglII ctagtAGATCTATGAAGGGGACAATCGTCGG

HNX-R Reverse primer anneals to XhoI cgtgaCTCGAGgaatgGGATCCTTAATTTTTCTTATACTCA