Template:BerkiGEM2007 HannahConstructionFiles
From 2007.igem.org
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[[BerkiGEM2007-HannahConstructionFile1 | Construction of I716316)]]<br> | [[BerkiGEM2007-HannahConstructionFile1 | Construction of I716316)]]<br> | ||
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| + | PCR HNX-F and HNX-R on H-Nox (567bp, BglII/XhoI) | ||
| + | Sub into pBca1144 ( BglII/XhoI, 2054+910 L) | ||
| + | Product is pBca1144-H-NOX | ||
| + | ---------------------------------------------------------- | ||
| + | HNX-F Forward primer anneals to BglII ctagtAGATCTATGAAGGGGACAATCGTCGG | ||
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| + | HNX-R Reverse primer anneals to XhoI cgtgaCTCGAGgaatgGGATCCTTAATTTTTCTTATACTCA | ||
Revision as of 21:30, 28 June 2007
PCR HNX-F and HNX-R on H-Nox (567bp, BglII/XhoI) Sub into pBca1144 ( BglII/XhoI, 2054+910 L) Product is pBca1144-H-NOX
HNX-F Forward primer anneals to BglII ctagtAGATCTATGAAGGGGACAATCGTCGG
HNX-R Reverse primer anneals to XhoI cgtgaCTCGAGgaatgGGATCCTTAATTTTTCTTATACTCA
