BerkiGEM2007-AustinConstructionFile25
From 2007.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
Beta portion<br> | Beta portion<br> | ||
pcr CA1154F/G00101 on 9145-I716063 (575 bp)<br> | pcr CA1154F/G00101 on 9145-I716063 (575 bp)<br> | ||
| - | Product is | + | Product is ADtemp1b<br> |
| - | Sub | + | Sub ADtemp1b into pTRC plasmid with (Bgl2/XhoI)<br> |
| - | Product is | + | Product is ADtemp2b<br> |
| - | pcr CA1099F/D58 on | + | pcr CA1099F/D58 on ADtemp2b (3465 bp)<br> |
| - | Product is | + | Product is ADtemp3b<br> |
_______________________________________________________<br> | _______________________________________________________<br> | ||
Alpha portion<br> | Alpha portion<br> | ||
Pcr CA1150F/CA1099R on I716073 (1727 bp)<br> | Pcr CA1150F/CA1099R on I716073 (1727 bp)<br> | ||
| - | Product is | + | Product is ADtemp5b<br> |
____________________________________________________________<br> | ____________________________________________________________<br> | ||
| - | Digest | + | Digest ADtemp3b (BsaI/BamHI)<br> |
| - | Digest | + | Digest ADtemp5b (BsaI/BglII)<br> |
Ligate, product is: I716075 (Best all around) | Ligate, product is: I716075 (Best all around) | ||
<br> | <br> | ||
Revision as of 22:51, 30 June 2007
Beta portion
pcr CA1154F/G00101 on 9145-I716063 (575 bp)
Product is ADtemp1b
Sub ADtemp1b into pTRC plasmid with (Bgl2/XhoI)
Product is ADtemp2b
pcr CA1099F/D58 on ADtemp2b (3465 bp)
Product is ADtemp3b
_______________________________________________________
Alpha portion
Pcr CA1150F/CA1099R on I716073 (1727 bp)
Product is ADtemp5b
____________________________________________________________
Digest ADtemp3b (BsaI/BamHI)
Digest ADtemp5b (BsaI/BglII)
Ligate, product is: I716075 (Best all around)
