Melb:Primary

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Revision as of 08:04, 1 July 2007

Contents

Primary Reagents & Disposables

Primary Reagents

Kits

Miniprep kit Gel clean-up system

Enzymes

Restriction enzymes (in particular, NotI, EcoRI, XbaI, HindIII) Ligase Competent cells (both for plasmid purification and protein expression) DNA standard

Chemicals

Ingredients for media (LB, SOB) Glycerol Buffers (including TAE and TE) Loading dye for DNA gel

Disposables

TUBES:

Falcon: 10ml, 50ml microcentrifuge: 1.7ml

Tips

Tips (10, 200, 1000 ul)

Other

Gloves (Large , Med, Small) Scalpel Tube racks Freezer boxes Parafilm