Melb:Primary
From 2007.igem.org
(Difference between revisions)
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==Primary Reagents== | ==Primary Reagents== | ||
===Kits=== | ===Kits=== | ||
- | Miniprep kit | + | *Miniprep kit |
- | Gel clean-up system | + | *Gel clean-up system |
===Enzymes=== | ===Enzymes=== | ||
- | Restriction enzymes (in particular, NotI, EcoRI, XbaI, HindIII) | + | *Restriction enzymes (in particular, NotI, EcoRI, XbaI, HindIII) |
- | Ligase | + | *Ligase |
- | Competent cells (both for plasmid purification and protein expression) | + | *Competent cells (both for plasmid purification and protein expression) |
- | DNA standard | + | *DNA standard |
===Chemicals=== | ===Chemicals=== | ||
- | Ingredients for media (LB, SOB) | + | *Ingredients for media (LB, SOB) |
- | Glycerol | + | *Glycerol |
- | Buffers (including TAE and TE) | + | *Buffers (including TAE and TE) |
- | Loading dye for DNA gel | + | *Loading dye for DNA gel |
==Disposables== | ==Disposables== | ||
===TUBES:=== | ===TUBES:=== | ||
- | Falcon: 10ml, 50ml | + | *Falcon: 10ml, 50ml |
- | microcentrifuge: 1.7ml | + | *microcentrifuge: 1.7ml |
===Tips=== | ===Tips=== | ||
- | Tips (10, 200, 1000 ul) | + | *Tips (10, 200, 1000 ul) |
===Other=== | ===Other=== | ||
- | Gloves (Large , Med, Small) | + | *Gloves (Large , Med, Small) |
- | + | *Scalpels | |
- | Tube racks | + | *Tube racks |
- | Freezer boxes | + | *Freezer boxes |
- | Parafilm | + | *Parafilm |
Revision as of 08:06, 1 July 2007
Contents |
Primary Reagents & Disposables
Primary Reagents
Kits
- Miniprep kit
- Gel clean-up system
Enzymes
- Restriction enzymes (in particular, NotI, EcoRI, XbaI, HindIII)
- Ligase
- Competent cells (both for plasmid purification and protein expression)
- DNA standard
Chemicals
- Ingredients for media (LB, SOB)
- Glycerol
- Buffers (including TAE and TE)
- Loading dye for DNA gel
Disposables
TUBES:
- Falcon: 10ml, 50ml
- microcentrifuge: 1.7ml
Tips
- Tips (10, 200, 1000 ul)
Other
- Gloves (Large , Med, Small)
- Scalpels
- Tube racks
- Freezer boxes
- Parafilm