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Revision as of 08:17, 1 July 2007 (view source) PhillipDodson (Talk | contribs) ← Older edit		Revision as of 08:18, 1 July 2007 (view source) PhillipDodson (Talk | contribs) Newer edit →
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	*Falcon: 10ml, 50ml		*Falcon: 10ml, 50ml
	*microcentrifuge: 1.7ml		*microcentrifuge: 1.7ml
		+	*PCR
	===Tips===		===Tips===
	*Tips (10, 200, 1000 ul)		*Tips (10, 200, 1000 ul)

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Revision as of 08:18, 1 July 2007

Contents 1 Primary Reagents & Disposables 1.1 Primary Reagents 1.1.1 Kits 1.1.2 Enzymes 1.1.3 Chemicals 1.2 Disposables 1.2.1 TUBES: 1.2.2 Tips 1.2.3 Other

Primary Reagents & Disposables

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Primary Reagents

Kits

• Miniprep kit
• Gel clean-up system

Enzymes

• Restriction enzymes (in particular, NotI, EcoRI, XbaI, HindIII)
• Ligase
• Competent cells (both for plasmid purification and protein expression)
• DNA standard

Chemicals

• Ingredients for media (LB, SOB)
• Glycerol
• Buffers (including TAE and TE)
• Loading dye for DNA gel

Disposables

TUBES:

• Falcon: 10ml, 50ml
• microcentrifuge: 1.7ml
• PCR

Tips

• Tips (10, 200, 1000 ul)

Other

• Gloves (Large , Med, Small)
• Scalpels
• Tube racks
• Freezer boxes
• Parafilm