BerkiGEM2007-AustinConstructionFile25
From 2007.igem.org
(Difference between revisions)
| Line 4: | Line 4: | ||
Sub ADtemp1b into pTRC plasmid with (Bgl2/XhoI)<br> | Sub ADtemp1b into pTRC plasmid with (Bgl2/XhoI)<br> | ||
Product is ADtemp2b<br> | Product is ADtemp2b<br> | ||
| - | pcr | + | pcr CA1099R/D58 on ADtemp2b (3465 bp)<br> |
Product is ADtemp3b<br> | Product is ADtemp3b<br> | ||
_______________________________________________________<br> | _______________________________________________________<br> | ||
Revision as of 02:13, 2 July 2007
Beta portion
pcr CA1154F/G00101 on 9145-I716063 (575 bp)
Product is ADtemp1b
Sub ADtemp1b into pTRC plasmid with (Bgl2/XhoI)
Product is ADtemp2b
pcr CA1099R/D58 on ADtemp2b (3465 bp)
Product is ADtemp3b
_______________________________________________________
Alpha portion
Pcr CA1150F/CA1099R on I716077 ()
Product is ADtemp5b
____________________________________________________________
Digest ADtemp3b (BsaI/BamHI)
Digest ADtemp5b (BsaI/BglII)
Ligate, product is: I716075 (Best all around)
