ThpA
From 2007.igem.org
(Difference between revisions)
Revision as of 17:53, 2 July 2007
PCR ThpA F/R on ThpA (553bp, BglII/XhoI)
Sub into PBca1144 (BglII/XhoI, 2054+910, L)
Product is pBca9145-BBa I716331
ThpAF Forward BglII site anneals to ThpA cgtacAGATCTatgaccgccgcaccagcagattttgcccgtgcccgaagcgaGttcctcagtatcg
ThpAR Reverse XhoI site anneals to ThpA anneals to XhoI cgcatctcgagtcaggtggcgaacgggccta
