ThpA
From 2007.igem.org
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PCR ThpA F/R on ThpA (553bp, BglII/XhoI)<br> | PCR ThpA F/R on ThpA (553bp, BglII/XhoI)<br> | ||
| - | Sub into | + | Sub into pBca9145-Bca1144 (BglII/XhoI, 2054+910, L)<br> |
Product is pBca9145-BBa I716331 <br> | Product is pBca9145-BBa I716331 <br> | ||
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Latest revision as of 18:04, 2 July 2007
PCR ThpA F/R on ThpA (553bp, BglII/XhoI)
Sub into pBca9145-Bca1144 (BglII/XhoI, 2054+910, L)
Product is pBca9145-BBa I716331
ThpAF Forward BglII site anneals to ThpA cgtacAGATCTatgaccgccgcaccagcagattttgcccgtgcccgaagcgaGttcctcagtatcg
ThpAR Reverse XhoI site anneals to ThpA anneals to XhoI cgcatctcgagtcaggtggcgaacgggccta
