Melbourne/Lab Notebook
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==Week 1== | ==Week 1== | ||
===25 June 2007=== | ===25 June 2007=== | ||
| + | |||
| + | Prepared LB agar plates. | ||
| + | |||
| + | ====Transformation==== | ||
| + | #resuspended the following from Registry plates: | ||
| + | **BBa_I15008 - ho1 (P2 21A) | ||
| + | **BBa_I15009 - PcyA (P2 21C) | ||
| + | **BBa_R0084 - OmpR positive promoter (P1 11H) | ||
| + | ##Punctured foil with pipette tip. | ||
| + | ##Resuspended in 15uL ddH2O. | ||
| + | ##Stored in-20 (after taking 1uL for transformation). | ||
| + | #[[Melb:Transformation Protocol|Transformed]] into competent DH5alpha cells. | ||
| + | |||
===26 June 2007=== | ===26 June 2007=== | ||
===27 June 2007=== | ===27 June 2007=== | ||
Revision as of 00:25, 3 July 2007
Contents |
Week 1
25 June 2007
Prepared LB agar plates.
Transformation
- resuspended the following from Registry plates:
- BBa_I15008 - ho1 (P2 21A)
- BBa_I15009 - PcyA (P2 21C)
- BBa_R0084 - OmpR positive promoter (P1 11H)
- Punctured foil with pipette tip.
- Resuspended in 15uL ddH2O.
- Stored in-20 (after taking 1uL for transformation).
- Transformed into competent DH5alpha cells.
