Melbourne/Lab Notebook
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##Resuspended in 15uL ddH2O. | ##Resuspended in 15uL ddH2O. | ||
##Stored in-20 (after taking 1uL for transformation). | ##Stored in-20 (after taking 1uL for transformation). | ||
| - | #[[Melb:Transformation Protocol|Transformed]] into competent DH5alpha cells | + | #[[Melb:Transformation Protocol|Transformed]] into competent DH5alpha cells with shorter incubationtimes as follows: |
| + | **30min on ice after DNA addition | ||
| + | **10min on ice after heat shock | ||
| + | **30min at 37degrees with LB | ||
===26 June 2007=== | ===26 June 2007=== | ||
Revision as of 00:32, 3 July 2007
Contents |
Week 1
25 June 2007
Prepared LB agar plates.
Transformation
- resuspended the following from Registry plates:
- BBa_I15008 - ho1 (P2 21A)
- BBa_I15009 - PcyA (P2 21C)
- BBa_R0084 - OmpR positive promoter (P1 11H)
- Punctured foil with pipette tip.
- Resuspended in 15uL ddH2O.
- Stored in-20 (after taking 1uL for transformation).
- Transformed into competent DH5alpha cells with shorter incubationtimes as follows:
- 30min on ice after DNA addition
- 10min on ice after heat shock
- 30min at 37degrees with LB
