Melbourne/Lab Notebook
From 2007.igem.org
(Difference between revisions)
(→Transformation) |
(→26 June 2007) |
||
| Line 19: | Line 19: | ||
===26 June 2007=== | ===26 June 2007=== | ||
| + | |||
| + | ====Transformation from Monday==== | ||
| + | *Transformation of BBa_I15008 and BBa_I15009 failed. No colonies on plates | ||
| + | *Small number of colonies on BBa_R0084 plate. | ||
| + | *Placed in cool room | ||
| + | |||
===27 June 2007=== | ===27 June 2007=== | ||
===28 June 2007=== | ===28 June 2007=== | ||
Revision as of 00:45, 3 July 2007
Contents |
Week 1
25 June 2007
Prepared LB agar plates.
Transformation
- resuspended the following from Registry plates:
- BBa_I15008 - ho1 (P2 21A)
- BBa_I15009 - PcyA (P2 21C)
- BBa_R0084 - OmpR positive promoter (P1 11H)
- Punctured foil with pipette tip.
- Resuspended in 15uL ddH2O.
- Stored in-20 (after taking 1uL for transformation).
- Transformed into competent DH5alpha cells with shorter incubation times as follows:
- 30min on ice after DNA addition
- 10min on ice after heat shock
- 30min at 37degrees with LB
26 June 2007
Transformation from Monday
- Transformation of BBa_I15008 and BBa_I15009 failed. No colonies on plates
- Small number of colonies on BBa_R0084 plate.
- Placed in cool room
