From 2007.igem.org

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Revision as of 12:26, 3 July 2007

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Experiments

• pL.LuxI.C was inoculated in 3 mL L/B and 3 uL 100 mg/mL Amp for equivalence expt to be done on July 2.

• pL.LuxR.Y was inoculated in 3 mL L/B and 3 uL 100 mg/mL Amp for equivalence expt to be done on July 2.

• Dilutions of pL.LuxI.C made in M9 with IPTG conc 0,5,10,50,100,500,1000 uM

i) for FACS (in triplicate for a particular inoculum volume) ii) for Microscopy (for 2 inoculum volumes: 0.1 & 0.01 uL/mL)

• Dilutions of pL.LuxR.Y made in M9 with IPTG conc 0,5,10,50,100,500,1000 uM

i) for FACS (in triplicate) ii) for Microscopy (duplicate).