Wisconsin/Protocol:Ligation

From 2007.igem.org

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(Ligation)
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===Ligation===
===Ligation===
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Use Nanospectrometer to find vector and insert concentration in [ng/uL]. Calculate the amount of vector and insert to use with this excel spreadsheet.
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Use Nanodrop to find vector and insert concentration in [ng/uL]. Find the length of vector and insert and calculate volume of reagents needed with [http://mywebspace.wisc.edu/klapoetke/web/iGEM/WIDGET_Ligation_Reaction.xls this excel spreadsheet].

Revision as of 23:27, 5 July 2007

Ligation

Use Nanodrop to find vector and insert concentration in [ng/uL]. Find the length of vector and insert and calculate volume of reagents needed with [http://mywebspace.wisc.edu/klapoetke/web/iGEM/WIDGET_Ligation_Reaction.xls this excel spreadsheet].