Wisconsin/Protocol:Ligation
From 2007.igem.org
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| - | Use | + | Use Nanodrop to find vector and insert concentration in [ng/uL]. Find the length of vector and insert and calculate volume of reagents needed with [http://mywebspace.wisc.edu/klapoetke/web/iGEM/WIDGET_Ligation_Reaction.xls this excel spreadsheet]. |
Revision as of 23:27, 5 July 2007
Ligation
Use Nanodrop to find vector and insert concentration in [ng/uL]. Find the length of vector and insert and calculate volume of reagents needed with [http://mywebspace.wisc.edu/klapoetke/web/iGEM/WIDGET_Ligation_Reaction.xls this excel spreadsheet].
