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Revision as of 09:55, 6 July 2007

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Experiments

1000: [5'] Transfer to 50 mL Glu M9 in 250 mL flask with [aTc]=5 ng/mL and inoculum=1,0.1 uL/mL

                          (Navneet)               (20*50=1 ug == 25 uL of 10 ug/mL) 

1000: [1'] Transfer to 50 mL Glu M9 in 250 mL flask with [aTc]=1 ng/mL and inoculum=1,0.1 uL/mL

                          (Navneet)               (10*50=0.5 ug == 5 uL of 10 ug/mL aTc)

1040: Inoculate K12z1 in 5 mL Glu M9 in 50 mL tube with [aTc]=5 ng/mL and inoculum=0.5,0.05 uL/mL

                                        (20*5=0.1 ug == 2.5 uL of 10 ug/mL)
   [5']  (Navneet)

1040: Inoculate K12z1 in 5 mL Glu M9 in 50 mL tube with [aTc]=1 ng/mL and inoculum=0.5,0.05 uL/mL

                                        (10*5=0.05 ug == 0.5 uL of 10 ug/mL)
   [1']  (Navneet)

1145: [20'] Image Open loop samples

1230: Inoculate pL.LuxR.Y.pR.C, K12z1, pL.Y in LB (Navneet)

1400: [0] Image Open loop samples

1530: Inoculate pL.LuxR.Y.pR.C, K12z1 in LB (Vivek)

2315: [1'] Filtered OD flask=0.193 (Navneet)

     (5' has to be repeated; right OD was not reached in time)

0030: Made 7 dilutions for pL.LuxR.Y.pR.C with AI from 1 ng/mL aTc and new 2xM9 in 1:1 ratio

     [1']             -ve control: K12z1 in AI from 1 ng/mL aTc and new 2xM9 in 1:1 ratio                                                         +ve control: pL.Y, 100 uM IPTG, 1x M9    (Krishna)   
                             

0105: [50'],[5'] Inoculated pT.LuxI.C, K12Z1 in L/B

0145: [1'] Only M9, 500 uM IPTG, pL.LuxR.Y.pR.C

            K12Z1-grown in aTc 1 medium:2x M9, 500 uM IPTG, pL.LuxR.Y.pR.C