July 6
From 2007.igem.org
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| - | |[[Image:Openloop_cfp_jul6.jpg|thumb| | + | |[[Image:Openloop_cfp_jul6.jpg|thumb|300px|CFP fluorescence]] |
| - | |[[Image:Openloop_yfp_jul6.jpg|thumb| | + | |[[Image:Openloop_yfp_jul6.jpg|thumb|300px|YFP fluorescence]] |
|- | |- | ||
| - | |[[Image:surf_plot_cfp_jul6.jpg|thumb| | + | |[[Image:surf_plot_cfp_jul6.jpg|thumb|300px|CFP fluorescence]] |
| - | |[[Image:surf_plot_yfp_jul6.jpg|thumb| | + | |[[Image:surf_plot_yfp_jul6.jpg|thumb|300px|YFP fluorescence]] |
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|colspan="2"| | |colspan="2"| | ||
The increasing values along the luxI.CFP axis are the increasing concentrations of aTc whereas the increasing values along the luxR.YFP axis are the increasing concentrations of IPTG. | The increasing values along the luxI.CFP axis are the increasing concentrations of aTc whereas the increasing values along the luxR.YFP axis are the increasing concentrations of IPTG. | ||
|} | |} | ||
Revision as of 06:07, 7 July 2007
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Experiments
Analysis
Today we compiled the data collected from all our open-loop experiments performed at various concentrations of aTc (ng/ml). The following matrix of information was obtained:
|
CFP mean values |
IPTG (uM) or luxR.YFP | |||||||
|---|---|---|---|---|---|---|---|---|
| 0 | 5 | 10 | 50 | 100 | 500 | 1000 | ||
|
aTc (ng/ml) or luxI.CFP | 0 | |||||||
| 1 | ||||||||
| 5 | ||||||||
| 10 | ||||||||
| 20 | ||||||||
| 50 | ||||||||
|
YFP mean values |
IPTG (uM) or luxR.YFP | |||||||
|---|---|---|---|---|---|---|---|---|
| 0 | 5 | 10 | 50 | 100 | 500 | 1000 | ||
|
aTc (ng/ml) or luxI.CFP | 0 | |||||||
| 1 | ||||||||
| 5 | ||||||||
| 10 | ||||||||
| 20 | ||||||||
| 50 | ||||||||
| These values, obtained from the microscopy experiments, give us a clear idea of the equivalence between luxI.CFP and luxI.YFP.
A scatter plot with each dot representing a cell was also plotted to see the variation of fluorescence with the change in aTc and IPTG concentrations.
|
 |  |
 |  |
|
The increasing values along the luxI.CFP axis are the increasing concentrations of aTc whereas the increasing values along the luxR.YFP axis are the increasing concentrations of IPTG. | |
