July 6
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Revision as of 06:15, 7 July 2007
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Experiments
Analysis
Today we compiled the data collected from all our open-loop experiments performed at various concentrations of aTc (ng/ml). The following matrix of information was obtained:
CFP mean values |
IPTG (uM) or luxR.YFP | |||||||
---|---|---|---|---|---|---|---|---|
0 | 5 | 10 | 50 | 100 | 500 | 1000 | ||
aTc (ng/ml) or luxI.CFP | 0 | |||||||
1 | ||||||||
5 | ||||||||
10 | ||||||||
20 | ||||||||
50 |
YFP mean values |
IPTG (uM) or luxR.YFP | |||||||
---|---|---|---|---|---|---|---|---|
0 | 5 | 10 | 50 | 100 | 500 | 1000 | ||
aTc (ng/ml) or luxI.CFP | 0 | |||||||
1 | ||||||||
5 | ||||||||
10 | ||||||||
20 | ||||||||
50 |
These values, obtained from the microscopy experiments, give us a clear idea of the equivalence between luxI.CFP and luxI.YFP.
A scatter plot with each dot representing a cell was also plotted to see the variation of fluorescence with the change in aTc and IPTG concentrations.
|
The increasing values along the luxI.CFP axis are the increasing concentrations of aTc whereas the increasing values along the luxR.YFP axis are the increasing concentrations of IPTG. |