July 6
From 2007.igem.org
(Difference between revisions)
(→Analysis) |
(→Analysis) |
||
| Line 11: | Line 11: | ||
Today we compiled the data collected from all our open-loop experiments performed at various concentrations of aTc (ng/ml). | Today we compiled the data collected from all our open-loop experiments performed at various concentrations of aTc (ng/ml). | ||
The following matrix of information was obtained: | The following matrix of information was obtained: | ||
| - | {|cellspacing=" | + | {|cellspacing="0" cellpadding="3" border="1" align="left" |
|-align="center" | |-align="center" | ||
!colspan="2" rowspan="2"| | !colspan="2" rowspan="2"| | ||
| Line 88: | Line 88: | ||
| | | | ||
|} | |} | ||
| - | {|cellspacing=" | + | {|cellspacing="0" cellpadding="3" border="1" align="right" |
|-align="center" | |-align="center" | ||
!colspan="2" rowspan="2"| | !colspan="2" rowspan="2"| | ||
| Line 165: | Line 165: | ||
| | | | ||
|} | |} | ||
| - | {|align="center" width=" | + | {|align="center" width="40%" |
|- | |- | ||
|align="justify"|These values, obtained from the microscopy experiments, give us a clear idea of the equivalence between luxI.CFP and luxI.YFP. | |align="justify"|These values, obtained from the microscopy experiments, give us a clear idea of the equivalence between luxI.CFP and luxI.YFP. | ||
Revision as of 06:38, 7 July 2007
Back to Bangalore
Back to e-Notebook
Experiments
Analysis
Today we compiled the data collected from all our open-loop experiments performed at various concentrations of aTc (ng/ml). The following matrix of information was obtained:
|
CFP mean values |
IPTG (uM) or luxR.YFP | |||||||
|---|---|---|---|---|---|---|---|---|
| 0 | 5 | 10 | 50 | 100 | 500 | 1000 | ||
|
aTc (ng/ml) or luxI.CFP | 0 | |||||||
| 1 | ||||||||
| 5 | ||||||||
| 10 | ||||||||
| 20 | ||||||||
| 50 | ||||||||
|
YFP mean values |
IPTG (uM) or luxR.YFP | |||||||
|---|---|---|---|---|---|---|---|---|
| 0 | 5 | 10 | 50 | 100 | 500 | 1000 | ||
|
aTc (ng/ml) or luxI.CFP | 0 | |||||||
| 1 | ||||||||
| 5 | ||||||||
| 10 | ||||||||
| 20 | ||||||||
| 50 | ||||||||
| These values, obtained from the microscopy experiments, give us a clear idea of the equivalence between luxI.CFP and luxI.YFP.
A scatter plot with each dot representing a cell was also plotted to see the variation of fluorescence with the change in aTc and IPTG concentrations.
|
| CFP Fluorescence | YFP Fluorescence |
|---|---|
| 
|
| 
|
|
The increasing values along the luxI.CFP axis are the increasing concentrations of aTc whereas the increasing values along the luxR.YFP axis are the increasing concentrations of IPTG. | |
