Melbourne/BBa B0014

From 2007.igem.org

(Difference between revisions)
(Expected Digestion Products)
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{| border="1"
{| border="1"
|+ Expected Digestion Products
|+ Expected Digestion Products
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! Label !! Registry Part Number !! Part Name !! Part Size(bp) !! Plasmid Vector !! Plasmid Size(bp) !! Total size(bp) !! Resistance  !! Status
+
! Digest !! Fragment 1 !! Fragment 2 !! Fragment 3
|-
|-
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! [[Melbourne/BBa_B0014|P1 1G]]
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! EcoRI/SpeI
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| BBa_B0014 || Double Terminator || 95 || pSB1AK3 || 3189 || 3284 || Amp, Kan || Confirmed
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| 131 || 3058
|-
|-
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! [[Melbourne/BBa_B0034|P1 3O]]
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! EcoRI/HaeII
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| BBa_B0034 || RBS || 12 || pSB1A2 || 2079 || 2091 || Amp || Miniprepped/Unconfirmed
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| 579|| 2705
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|-
+
 
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! [[Melbourne/BBa_C0051|P1 5G]]
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| BBa_C0051 || c1 represser protein || 750 || pSB1A2 || 2079 || 2892 || Amp || Miniprepped/Unconfirmed)
+
__NOTOC__
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Revision as of 04:15, 9 July 2007

<Return to Parts Used> <Return to Lab notebook> <Return to team homepage>

Name: Double Terminator

Last Updated: 10:20am 09/07/07

Physical DNA

  • Registry: BBa_0014
  • Kit location: P1 1G
  • Resistance: Amp, Kan
  • Part Size: 95
  • Plasmid Vector: pSB1AK3
  • Vector Size: 3189
  • Total Size: 3284

Status

  • Labelling: P1 1G
  • Resuspended: Yes
    • Location: -20 freezer
  • Transformed: Yes
    • Location: Cold Room
    • Miniprepped: 5/7
      • Location: -20 freezer
      • Confirmed: Yes
    • Glycerol Stock: 5/7
      • Location: -80 freezer

Expected Digestion Products

Expected Digestion Products
Digest Fragment 1 Fragment 2 Fragment 3
EcoRI/SpeI 131 3058
EcoRI/HaeII 579 2705