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	* BBa_J23100: strong constitutive promoter in BBa_J61002 (AmpR)		* BBa_J23100: strong constitutive promoter in BBa_J61002 (AmpR)
	* BBa_B0015: double terminator (B0010-B0012) in pSB1AK3 (AmpR)		* BBa_B0015: double terminator (B0010-B0012) in pSB1AK3 (AmpR)
		+	=== Caracterisation of w121 - MG1655 supplementation ===
		+	This part is just a preliminary work whose aim is to show that two strains can complement each other. A more detailed explanation of these experiments can be found here, here, and here.
		+	* Several conditions were tested but the curves are difficult to interpret.

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Revision as of 16:07, 13 July 2007

See the Notebook

Contents 1 What's new ? 2 To Do List 3 In Progress 3.1 FstZ strain caracterisation 3.2 Transduction of the deletion of DapA from the strain w121 into MG1655 and MG1655 FtsZ TS84 3.3 Transfection of 3 Biobricks 3.4 Caracterisation of w121 - MG1655 supplementation

What's new ?

We just received a first part of the oligos

To Do List

• Cloning of I0500 (inducible pBad) in [http://partsregistry.org/Part:BBa_J61002 J61002]

This will allow us to compare the strength of the pBad and the strengh of the constitutive promoter. Moreover, we'll be able to clone any gene we like after the promoter.

• Cloning of Lox71-FtsZ and Lox71-FtsA-FtsZ in J61002 with the constitutive promoter and with the pBad

This will allow us to see the effect of the overexpression of FtsZ and FtsA+FtsZ on the growth an phenotype. We expect the overexpression of FtsZ only to be lethal and the on of FtsA+FtsZ to form a viable mini-cell phenotype.

• Cloning of Lox66-DapA of Coli and Subtilis in the same 2 vectors

In Progress

FstZ strain caracterisation

We try to obtain a reliable TS clone, which would not grow at 42°C, but grow at 30°C

• Unsuccessful with the three clones we tested at the moment.

Transduction of the deletion of DapA from the strain w121 into MG1655 and MG1655 FtsZ TS84

The goal is, by transduction, to introduce DapA deletion into FtsZ TS strain

• We managed to build a stock of efficient phages (stock of 12.7.7)
• Transduction in progress

Transfection of 3 Biobricks

The goal is to extract DNA from the plates and make a stock of plasmid we need.

• BBa_I0500: Inducible pBad/araC in pSB2K3 (KanR)
• BBa_J23100: strong constitutive promoter in BBa_J61002 (AmpR)
• BBa_B0015: double terminator (B0010-B0012) in pSB1AK3 (AmpR)

Caracterisation of w121 - MG1655 supplementation

This part is just a preliminary work whose aim is to show that two strains can complement each other. A more detailed explanation of these experiments can be found here, here, and here.

• Several conditions were tested but the curves are difficult to interpret.