Experiments
From 2007.igem.org
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The following is the record of all the experiments done by us, each followed by graphs obtained by analysis of the corresponding microscopy and flow cytometry data. | The following is the record of all the experiments done by us, each followed by graphs obtained by analysis of the corresponding microscopy and flow cytometry data. | ||
| - | In flow cytometry a signal obtained from a filter does not exactly correspond to CFP or YFP amount inside a cell; when cells express both the proteins. This is because of spectral overlap of their excitation and emission spectra. We came up with a mathematical method to separate CFP and YFP from autofluorescence and noise. The details about this mathematical tool for correction can be found [[Media:Analysis.pdf|here]]. | + | In flow cytometry a signal obtained from a filter does not exactly correspond to CFP or YFP amount inside a cell; when cells express both the proteins. This is because of spectral overlap of their excitation and emission spectra. We came up with a mathematical method to separate CFP and YFP from autofluorescence and noise. The details about this mathematical tool for correction can be found [[Media:Analysis.pdf|here]].</font> |
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Revision as of 06:39, 14 July 2007
| The official wiki of the NCBS iGEM 2007 Team |
| [http://www.ncbs.res.in/ National Centre for Biological Sciences, Bangalore] |
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| Bangalore | The Team | The Mission | Experiments | e-Notebook |
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The following is the record of all the experiments done by us, each followed by graphs obtained by analysis of the corresponding microscopy and flow cytometry data. In flow cytometry a signal obtained from a filter does not exactly correspond to CFP or YFP amount inside a cell; when cells express both the proteins. This is because of spectral overlap of their excitation and emission spectra. We came up with a mathematical method to separate CFP and YFP from autofluorescence and noise. The details about this mathematical tool for correction can be found here. |
