Edinburgh/DivisionPopper
From 2007.igem.org
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[https://2007.igem.org/Edinburgh https://static.igem.org/mediawiki/2007/f/f5/800px-Edinburgh_City_15_mod.JPG] | [https://2007.igem.org/Edinburgh https://static.igem.org/mediawiki/2007/f/f5/800px-Edinburgh_City_15_mod.JPG] | ||
| - | + | == Bacterial counter == | |
| - | The idea is to use a | + | The idea is to use a variety of recombinases to delete or invert sections of DNA and use this to count or trigger the expression of genes. |
| - | + | ===Project Goal=== | |
The aim is to produce output as a function of cell division. This can have the added potential of performing programmed cell death after a determined number of cell divisions. We also hope to further analyse cell division and recombinase mechanisms. | The aim is to produce output as a function of cell division. This can have the added potential of performing programmed cell death after a determined number of cell divisions. We also hope to further analyse cell division and recombinase mechanisms. | ||
| - | + | ===Current Device Idea=== | |
[[Image:Division pulser.png|800px]] | [[Image:Division pulser.png|800px]] | ||
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This can then be hooked up to another device such as a counter. | This can then be hooked up to another device such as a counter. | ||
| - | This device realise on dif sites flipping only once per division. To test whether this actually happens or not we will build this smaller, simpler device to prove flipping occurs upon cell division and a second test to show whether it flips once or many times per division | + | This device realise on dif sites flipping only once per division. In it's current configuration, Promoter A is repressed by represser A which is continually being produced. at this time there is no represser B being produced. |
| + | |||
| + | When cell division occurs, the section of DNA between the two dif sites gets reversed. Initially there is no represser B present in the cell so promoter B is able to produce an output of PoPS. As time goes on represser B gets produced and 'turns off' promoter B. At the same time represser A is no longer being produced and degrades (?) so when the next division occurs, promoter A will be capable of producing a PoPS output and the process repeats. | ||
| + | |||
| + | ===Assumptions=== | ||
| + | * The Dif sites flip only once during cell division | ||
| + | * Repressors A and B are produced and degrade faster than the cell cycle | ||
| + | * Promoter B does not interfere with the production of represser A | ||
| + | |||
| + | ===Initial Experiments=== | ||
| + | |||
| + | To test whether this actually happens or not we will build this smaller, simpler device to prove flipping occurs upon cell division and a second test to show whether it flips once or many times per division | ||
Revision as of 12:13, 16 July 2007
https://static.igem.org/mediawiki/2007/f/f5/800px-Edinburgh_City_15_mod.JPG
Contents |
Bacterial counter
The idea is to use a variety of recombinases to delete or invert sections of DNA and use this to count or trigger the expression of genes.
Project Goal
The aim is to produce output as a function of cell division. This can have the added potential of performing programmed cell death after a determined number of cell divisions. We also hope to further analyse cell division and recombinase mechanisms.
Current Device Idea
This device should output a PoPS signal every time a cell division occurs This can then be hooked up to another device such as a counter.
This device realise on dif sites flipping only once per division. In it's current configuration, Promoter A is repressed by represser A which is continually being produced. at this time there is no represser B being produced.
When cell division occurs, the section of DNA between the two dif sites gets reversed. Initially there is no represser B present in the cell so promoter B is able to produce an output of PoPS. As time goes on represser B gets produced and 'turns off' promoter B. At the same time represser A is no longer being produced and degrades (?) so when the next division occurs, promoter A will be capable of producing a PoPS output and the process repeats.
Assumptions
- The Dif sites flip only once during cell division
- Repressors A and B are produced and degrade faster than the cell cycle
- Promoter B does not interfere with the production of represser A
Initial Experiments
To test whether this actually happens or not we will build this smaller, simpler device to prove flipping occurs upon cell division and a second test to show whether it flips once or many times per division
