Paris/July 12
From 2007.igem.org
(Difference between revisions)
Nicolas C. (Talk | contribs) (→Titration of bacteriophages P1) |
Nicolas C. (Talk | contribs) (→Culture of FtsZ TS) |
||
| Line 29: | Line 29: | ||
isolation of 6 clones of the 121.1 on LBA+tet incubated at 42°C + Culture ON at 30°C <br> | isolation of 6 clones of the 121.1 on LBA+tet incubated at 42°C + Culture ON at 30°C <br> | ||
If one clone do not grow at 42, we'll use it to do the transduction | If one clone do not grow at 42, we'll use it to do the transduction | ||
| + | See [[Paris/July_13#Test of Ftsz TS strain| result.]] | ||
Revision as of 14:54, 18 July 2007
Preparation of a stock of phage on w121:
David B:
- Filtration of the Cacl2 and MgSO4 stocks
- Launch a culture:
- 15ml LB
- 225µl Cacl2
- 450µl MgSO4
- 90µl DAP
- 150µl W121 from an ON culture
- Let grow until OD=0.2 (~1h)
- Add 100µl P1 (stock 1010/ml)
- Incubate 2h
- Add 200µl Chloroform
- Vortex, centrifuge (15min @ 3000rpm)
- Recover the supernatant + add 200µl Chloroform = Stock keep at 4°C
Titration of bacteriophages P1
Nicolas C.
We measure the strength of former P1 preparation :
- The stock prepared the 3.7.7
- The stock prepared the 8.7.7
- The stock prepared today (12.7.7)
See protocols for details.
Culture of FtsZ TS
David B
We want to isolate a good clone of the strain FstZ TS
From the plate of FtsZ TS84 121:
isolation of 6 clones of the 121.1 on LBA+tet incubated at 42°C + Culture ON at 30°C
If one clone do not grow at 42, we'll use it to do the transduction
See result.
