Paris/Freezer

From 2007.igem.org

(Difference between revisions)
(→Digestion Products)
(→Digestion Products)
Line 74: Line 74:
{|
{|
|- style="background: #ccccff;"
|- style="background: #ccccff;"
-
! colspan="5" style="background: #ccccff;" | Plasmids: MiniPrep Products
+
! colspan="5" style="background: #ccccff;" | Digestion Products
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|width=5%| Number
|width=5%| Number
Line 83: Line 83:
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |1
| style="background: #ccffcc;" |1
-
|  
+
|<bbpart>pJ23100</bbpart>
-
|  
+
|EcoR1
-
|  
+
|Spe1
-
|  
+
|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |2
| style="background: #ccffcc;" |2
-
|
+
|<bbpart>pJ23100</bbpart>
-
|
+
|Spe1
-
|
+
|Pst1
|
|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |3
| style="background: #ccffcc;" |3
-
|
+
|<bbpart>pJ23107</bbpart>
-
|
+
|EcoR1
-
|
+
|Spe1
|
|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |4
| style="background: #ccffcc;" |4
-
|
+
|<bbpart>pJ23107</bbpart>
-
|
+
|Spe1
-
|
+
|Pst1
|
|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |5
| style="background: #ccffcc;" |5
 +
|<bbpart>pSB2K3</bbpart>
 +
|Ecor1
 +
|Pst1
|
|
-
|
+
|- style="background: #cccccc;" 
-
|
+
| style="background: #ccffcc;" |6
 +
|pSB1AK3
 +
|Ecor1
 +
|Pst1
|
|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |6
| style="background: #ccffcc;" |6
-
|
+
|Lox66 DapA E.coli (PCR product)
-
|
+
|Ecor1
-
|
+
|Pst1
|
|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
-
| style="background: #ccffcc;" |7
+
| style="background: #ccffcc;" |6
-
|
+
|Lox66 DapA E.coli (PCR product)
-
|
+
|Xba1
-
|
+
|Pst1
|
|
|}
|}

Revision as of 16:16, 18 July 2007

Contents

PCR products

PCR products
Number Name Length Comments
1 Lox71-FtsA-FtsZ-1 1537
2 FtsZ-2 1088
3 DGAT-1
4 DGAT-2
5 Lox66-DapAColi 985
6 Lox66-DapASubtilis 979
7 Lox71-FtsZ 1260 EcoR1 site not mutated

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products
Number Name Description
1 pJ23100 Strong constitutive promoter in J61002 (clones 1 + 2)
2 pJ23107 Medium constitutive promoter in J61002 (clones 1 + 2)
3 B0015 Double terminator in pSB1AK3 (clones 1 + 2)
3 I0500 AraC pBAD in pSB2K3 (clones 1 + 2)

Digestion Products

Digestion Products
Number Name Enzyme 1 Enzyme 2 Description
1 pJ23100 EcoR1 Spe1
2 pJ23100 Spe1 Pst1
3 pJ23107 EcoR1 Spe1
4 pJ23107 Spe1 Pst1
5 pSB2K3 Ecor1 Pst1
6 pSB1AK3 Ecor1 Pst1
6 Lox66 DapA E.coli (PCR product) Ecor1 Pst1
6 Lox66 DapA E.coli (PCR product) Xba1 Pst1

Strains (glycerol stock)

  • MG1655
  • Acinetobacter ADP1
  • w121
  • p2C (CFP fluorescent)
  • p2Y (YFP fluorescent)
  • FtsZTS strains
    • MG1655 FtsZ -TS 84
    • 121.1
    • 121.2
    • 121.3
    • 121.4
    • 121.5
    • 121.6
    • 129
    • 1.129
    • DCR 14.1
    • DCR 14.2
    • DCR 14.3
  • DH5alpha pKs::DGAT
  • pJ23100 Clone1
  • pJ23100 Clone2
  • BBa_B0015 Clone1
  • BBa_B0015 Clone2
Retrieved from "http://2007.igem.org/wiki/index.php/Paris/Freezer"