Template:Paris PCR 0

From 2007.igem.org

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{|
{|
|- style="background: #ccccff;"
|- style="background: #ccccff;"
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! colspan="3" style="background: #ccccff;" | PCR : {{{Title}}}
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! colspan="7" style="background: #ccccff;" | PCR : {{{Title}}}
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
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|width= 33%|
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|width= 20%|
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|width= 33%|
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|width= 1%|
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|width= 33%|  
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|width= 5%|
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|- style="background: #cccccc;"
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|width= 5%|
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| style="background: #ccffcc;" | Buffer (5x)
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|width= 5%|
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|colspan="2" | {{{Buffer}}}
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|width= 1%|
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|- style="background: #cccccc;"
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|width= 40%|
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| style="background: #ccffcc;" | MgCl<sub>2</sub> 10µM
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|-
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|colspan="2" | {{{MgCl2}}}
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|style="background: #ffcccc;"| PCR Settings
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|- style="background: #cccccc;"
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|
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| style="background: #ccffcc;" | dNTP 10µM
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|style="background: #ccccff;"| Name
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|colspan="2" | {{{dNTP}}}
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|style="background: #ccffcc;" colspan = "2" |{{{name}}}
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|- style="background: #cccccc;"
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|
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| style="background: #ccffcc;" | oligoF 10µM
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|style="background: #ccccff;"| Expected size
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| {{{n_oligoF}}}
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|-
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| {{{v_oligoF}}}
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|style="background: #ccccff;"| Annealing (°C)
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|- style="background: #cccccc;"
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|
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| style="background: #ccffcc;" | oligoR 10µM
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|style="background: #ccccff;"| Buffer (5x)
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| {{{n_oligoR}}}
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| style="background: #ccffcc;" colspan = "2" |{{{buffer}}}
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| {{{v_oligoR}}}
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|
-
|- style="background: #cccccc;"
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|style="background: #ccffcc;"| {{{size}}}
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| style="background: #ccffcc;" | water
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|-
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|colspan="2" | {{{water}}}
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|style="background: #ccffcc;"| {{{annealing}}}
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|- style="background: #cccccc;"
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|
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| style="background: #ccffcc;" | polymerase
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|style="background: #ccccff;"| MgCl<sub>2</sub> 10µM
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|colspan="2" | {{{pol}}}
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| style="background: #ccffcc;" colspan = "2" |{{{mgcl2}}}
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|- style="background: #cccccc;"
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|
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| style="background: #ccffcc;" | DNA
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|style="background: #ccccff;"| Success
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|colspan="2" | {{{DNA}}}
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|-  
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|- style="background: #cccccc;"
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|style="background: #ccccff;"| Time Elongation
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| style="background: #ccffcc;" | Expected Size kpb
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|
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|colspan="2" | {{{Size}}}
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|style="background: #ccccff;"| dNTP 10µM
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|- style="background: #cccccc;"
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| style="background: #ccffcc;" colspan = "2" |{{{dntp}}}
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| style="background: #ccffcc;" | Success
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|
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|colspan="2" | {{{Success}}}
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|style="background: #ccffcc;"| {{{success}}}
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|-
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|style="background: #ccffcc;"| {{{elongation}}}
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|
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|style="background: #ccccff;"| Oligo F
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|style="background: #ccffcc;"| {{{n_oligo_f}}}
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|style="background: #ccffcc;"| {{{n_oligo_f}}}
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|
 +
|style="background: #ccccff;"| Image
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|-  
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|style="background: #ccccff;"| Number cycles
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|
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|style="background: #ccccff;"| Oligo R
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|style="background: #ccffcc;"| {{{n_oligo_r}}}
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|style="background: #ccffcc;"| {{{n_oligo_r}}}
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|
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|style="background: #ccffcc;" rowspan| [[Image:{{{image}}}|10px]]
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|-
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|style="background: #ccffcc;"| {{{cycles}}}
 +
|
 +
|style="background: #ccccff;"| Water
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| style="background: #ccffcc;" colspan = "2" |{{{water}}}
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|
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|style="background: #ccccff;"| Band (0=ladder)
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|-  
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|style="background: #ccccff;"| Comments
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|
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|style="background: #ccccff;"| Polymerase
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| style="background: #ccffcc;" colspan = "2" |{{{pol}}}
 +
|
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|style="background: #ccffcc;"| {{{band}}}
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|-
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|style="background: #ccffcc;"| {{{comment}}}
 +
|
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|style="background: #ccccff;"| DNA
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| style="background: #ccffcc;" colspan = "2" |{{{dna}}}
 +
|
|}
|}

Revision as of 16:24, 18 July 2007

PCR : {{{Title}}}
PCR Settings Name {{{name}}} Expected size
Annealing (°C) Buffer (5x) {{{buffer}}} {{{size}}}
{{{annealing}}} MgCl2 10µM {{{mgcl2}}} Success
Time Elongation dNTP 10µM {{{dntp}}} {{{success}}}
{{{elongation}}} Oligo F {{{n_oligo_f}}} {{{n_oligo_f}}} Image
Number cycles Oligo R {{{n_oligo_r}}} {{{n_oligo_r}}} [[Image:{{{image}}}|10px]]
{{{cycles}}} Water {{{water}}} Band (0=ladder)
Comments Polymerase {{{pol}}} {{{band}}}
{{{comment}}} DNA {{{dna}}}
Retrieved from "http://2007.igem.org/wiki/index.php/Template:Paris_PCR_0"