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Revision as of 17:22, 18 July 2007 (view source) David.bikard (Talk | contribs) (→Plasmids: MiniPrep Products) ← Older edit		Revision as of 17:23, 18 July 2007 (view source) David.bikard (Talk | contribs) (→Plasmids: MiniPrep Products) Newer edit →
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	|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>		|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
	|Strong constitutive promoter in J61002 (clones 1 + 2)		|Strong constitutive promoter in J61002 (clones 1 + 2)
-	|	+	| 15/07/07
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" |2		| style="background: #ccffcc;" |2
	|<bbpart>pJ23107</bbpart> in <bbpart>J61002</bbpart>		|<bbpart>pJ23107</bbpart> in <bbpart>J61002</bbpart>
	|Medium constitutive promoter in J61002 (clones 1 + 2)		|Medium constitutive promoter in J61002 (clones 1 + 2)
-	|	+	| 15/07/07
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" |3		| style="background: #ccffcc;" |3
	|<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart>		|<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart>
	|Double terminator (clones 1 + 2)		|Double terminator (clones 1 + 2)
-	| 17/07/07	+	| 16/07/07
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" |4		| style="background: #ccffcc;" |4
	|<bbpart>I0500</bbpart> in <bbpart>pSB2K3</bbpart>		|<bbpart>I0500</bbpart> in <bbpart>pSB2K3</bbpart>
	|AraC pBAD (clones 1 + 2)		|AraC pBAD (clones 1 + 2)
-	| 17/07/07	+	| 16/07/07
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" |5		| style="background: #ccffcc;" |5

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Revision as of 17:23, 18 July 2007

Contents 1 PCR products 2 Plasmids: MiniPrep Products 3 Digestion Products 4 Strains (glycerol stock)

PCR products

PCR products
Number	Name	Length	Comments
1	Lox71-FtsA-FtsZ-1	1537
2	FtsZ-2	1088
3	DGAT-1
4	DGAT-2
5	Lox66-DapAColi	985
6	Lox66-DapASubtilis	979
7	Lox71-FtsZ	1260	EcoR1 site not mutated

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products
Number	Name	Description	Date
1	pJ23100 in J61002	Strong constitutive promoter in J61002 (clones 1 + 2)	15/07/07
2	pJ23107 in J61002	Medium constitutive promoter in J61002 (clones 1 + 2)	15/07/07
3	B0015 in pSB1AK3	Double terminator (clones 1 + 2)	16/07/07
4	I0500 in pSB2K3	AraC pBAD (clones 1 + 2)	16/07/07
5	BBa_B0030 in pSB1A2	Strong RBS (clones 1 + 2)	18/07/07
6	BBa_E0422 in pSB1A2	ECFP (RBS+LVA+Term) (clones 1 + 2)	18/07/07
7	BBa_E0241 in pSB1A2	PoPs to GFP converter (clones 1 + 2)	18/07/07
8	BBa_E0840 in pSB1A2	gfp tri-part; strong rbs (clones 1 + 2)	18/07/07
9	BBa_J61047 in pSB1A2	Cre ORF (clones 1 + 2)	18/07/07

Digestion Products

Digestion Products
Number	Name	Enzyme 1	Enzyme 2	Description
1	pJ23100	EcoR1	Spe1
2	pJ23100	Spe1	Pst1
3	pJ23107	EcoR1	Spe1
4	pJ23107	Spe1	Pst1
5	pSB2K3	EcoR1	Pst1
6	pSB1AK3	EcoR1	Pst1
6	Lox66 DapA E.coli (PCR product)	EcoR1	Pst1
7	Lox66 DapA E.coli (PCR product)	Xba1	Pst1

Strains (glycerol stock)

• MG1655
• Acinetobacter ADP1
• w121
• p2C (CFP fluorescent)
• p2Y (YFP fluorescent)
• FtsZ^TS strains
  • MG1655 FtsZ -TS 84
  • 121.1
  • 121.2
  • 121.3
  • 121.4
  • 121.5
  • 121.6
  • 129
  • 1.129
  • DCR 14.1
  • DCR 14.2
  • DCR 14.3
• DH5alpha pKs::DGAT
• pJ23100 Clone1
• pJ23100 Clone2
• BBa_B0015 Clone1
• BBa_B0015 Clone2