From 2007.igem.org

Revision as of 16:47, 18 July 2007 (view source) Landrain (Talk | contribs) ← Older edit		Revision as of 17:35, 18 July 2007 (view source) Nicolas C. (Talk | contribs) Newer edit →
Line 28:		Line 28:
	==Digestion products==		==Digestion products==
	Photos !!!		Photos !!!
		+	== DAP solution contamination test ==
		+	Results :
		+	* 50µL of H<sub>2</sub>0 (control) OK
		+	* 50µL of aliquot DAP 50mM  (in use) OK
		+	* 50µL of DAP 1mM CONTAMINATED
		+	* 50µL of DAP 0.2 mM OK
		+	* 50µL of DAP 50mM OK

---

Revision as of 17:35, 18 July 2007

Contents 1 FtsZTS strain screening 2 Minipreps 3 Digestion products 4 DAP solution contamination test

FtsZ^TS strain screening

We want to test these strains :

• 121.4
• 121.5
• 121.6
• 129
• 1.129
• DCR 14.1
• DCR 14.2
• DCR 14.3

I made glycerol stock of these strains, stored in the freezer. Test :

• Spread these strains on two plates (preheated before spreading) :
  • One at 30°C
  • One at 42°C

The results tomorrow.

Minipreps

We have 2 clones for each plasmid :

• E0422
• E0241
• E0840
• B0030
• J61047

Elution within 50µL H2O
DNA is within the Miniprep box in the -20°C fridge.

Digestion products

Photos !!!

DAP solution contamination test

Results :

• 50µL of H₂0 (control) OK
• 50µL of aliquot DAP 50mM (in use) OK
• 50µL of DAP 1mM CONTAMINATED
• 50µL of DAP 0.2 mM OK
• 50µL of DAP 50mM OK