Edinburgh/Lab

From 2007.igem.org

(Difference between revisions)
(Lab Diary)
Line 17: Line 17:
==Lab Diary==
==Lab Diary==
-
Thu 12th July - PTG induced GFP revived
+
Thu 12th July  
 +
* PTG induced GFP revived ([http://partsregistry.org/Part:BBa_J04430 BBa_J04430])
:: Test biobrick revival process
:: Test biobrick revival process
-
Fri 13th July - Alfalfa Seeds Planted (in Agar)
+
Fri 13th July
-
:: Growing alfalfa plants, to get leaves from which to extract DNA for the caffeic acid-3-o-methyltransferase gene (part of the vanillin synthesis pathway)  
+
* Alfalfa Seeds Planted (in Agar)
 +
:: Growing alfalfa plants, to get leaves from which to extract DNA for the caffeic acid-3-o-methyltransferase gene (part of the vanillin synthesis pathway)
 +
* Biobricks  [http://partsregistry.org/Part:BBa_E0241 BBa_E0241] and [http://partsregistry.org/Part:BBa_J45700 BBa_J45700] rivived
-
Mon 16th July - Begin DNA extraction from Bamboo
+
Mon 16th July  
 +
* Begin DNA extraction from Bamboo
:: Test DNA extraction process from plants
:: Test DNA extraction process from plants
 +
* Prepared mini preps for GFP and mint genes
-
Tue 17th July - Alfalfa Transferred to compost
+
Tue 17th July  
 +
* Alfalfa Transferred to compost
 +
 
 +
* DNA containing GFP and mint genes extracted from E.Coli

Revision as of 10:01, 19 July 2007

https://static.igem.org/mediawiki/2007/f/f5/800px-Edinburgh_City_15_mod.JPG

Biobricks Revived

This is a list of the biobricks we have revived from the registry

Brick Description Well Used for
[http://partsregistry.org/Part:BBa_J04430 BBa_J04430] IPTG induced GFP Plate 2, 1L Test biobrick revival process
[http://partsregistry.org/Part:BBa_E0241 BBa_E0241] PoPS --> GFP Plate 2, 15L For use in dif experement 1
[http://partsregistry.org/Part:BBa_J45700 BBa_J45700] Wintergreen Scent Plate 3, 7B Place into lacto bacillus

Lab Diary

Thu 12th July

* PTG induced GFP revived ([http://partsregistry.org/Part:BBa_J04430 BBa_J04430])
Test biobrick revival process

Fri 13th July

* Alfalfa Seeds Planted (in Agar)
Growing alfalfa plants, to get leaves from which to extract DNA for the caffeic acid-3-o-methyltransferase gene (part of the vanillin synthesis pathway)
* Biobricks  [http://partsregistry.org/Part:BBa_E0241 BBa_E0241] and [http://partsregistry.org/Part:BBa_J45700 BBa_J45700] rivived

Mon 16th July

* Begin DNA extraction from Bamboo
Test DNA extraction process from plants
* Prepared mini preps for GFP and mint genes

Tue 17th July

* Alfalfa Transferred to compost
* DNA containing GFP and mint genes extracted from E.Coli