Paris/Modeling char

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(Difference between revisions)
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* The promoters:
* The promoters:
-
#-DAPap: we must study the basic pops when no DAP is present, and establish the pops output curve depending of             
+
-DAPap: we must study the basic pops when no DAP is present, and establish the pops output curve depending of             
repression. look the arabinose AraC  
repression. look the arabinose AraC  
-
#-the high constitutive promoter that we are using:<bbpart>BBa_J23112</bbpart>
+
-the high constitutive promoter that we are using:<bbpart>BBa_J23112</bbpart>
*cre efficience :
*cre efficience :

Revision as of 13:38, 19 July 2007

We must characterise the different part we are using. Essentially :

  • The promoters:

-DAPap: we must study the basic pops when no DAP is present, and establish the pops output curve depending of repression. look the arabinose AraC

-the high constitutive promoter that we are using:BBa_J23112

  • cre efficience :


BBa_J5528 :Arabinose -> pBad = GFP : use it to test pbad efficiency,or make a similar construct

assemble Arabinose and cre one one plasmide, loxP ccdb loxP on another, and test the recombination frequency


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