| Plasmids: MiniPrep Products
|
| Number
| Name
| Description
| Product Left ?
| Date
|
| MP1
| pJ23100 in J61002
| Strong constitutive promoter in J61002 (clones 1 + 2)
|
| 15/07/07
|
| MP2
| pJ23107 in J61002
| Medium constitutive promoter in J61002 (clones 1 + 2)
| MP2.2
| 15/07/07
|
| MP3
| B0015 in pSB1AK3
| Double terminator (clones 1 + 2)
|
| 16/07/07
|
| MP4
| I0500 in pSB2K3
| AraC pBAD (clones 1 + 2)
|
| 16/07/07
|
| MP5
| BBa_B0030 in pSB1A2
| Strong RBS (clones 1 + 2)
|
| 18/07/07
|
| MP6
| BBa_E0422 in pSB1A2
| ECFP (RBS+LVA+Term) (clones 1 + 2)
|
| 18/07/07
|
| MP6'
| BBa_E0422 in pSB1A2
| ECFP (RBS+LVA+Term) (clones 1 + 2)
|
| 24/07/07
|
| MP7
| BBa_E0241 in pSB1A2
| PoPs to GFP converter (clones 1 + 2)
|
| 18/07/07
|
| MP7'
| BBa_E0241 in pSB1A2
| PoPs to GFP converter (clones 1)
|
| 24/07/07
|
| MP8
| BBa_E0840 in pSB1A2
| gfp tri-part; strong rbs (clones 1 + 2)
|
| 18/07/07
|
| MP9
| BBa_J61047 in pSB1A2
| Cre ORF (clones 1 + 2)
|
| 18/07/07
|
| MP9'
| BBa_J61047 in pSB1A2
| Cre ORF (clones 1 + 2)
|
| 24/07/07
|
| MP10
| BBa_I0500 in pSB2K3
| AraC pBAD (clones 1 + 2)
|
| 20/07/07
|
| MP11
| BBa_pJ23107 in p61002
| Medium constitutive promoter in J61002 (clones 3 + 4)
| MP11.3
| 20/07/07
|
| MP11'
| BBa_pJ23107 in p61002
| Medium constitutive promoter in J61002 (clones 3)
|
| 23/07/07
|
| Digestion Products
|
| Number
| Product Name
| Matrix Name
| Enzyme 1
| Enzyme 2
| Size
| Description
| Product Left
| Date
|
| D1
| pJ23100
| pJ23100
| Spe1
| Pst1
| 2096
| Vector with the J23100 promoter ready for insertion of a RBS/CDS insert
|
| 17/07/07
|
| D2
| mRFP
| pJ23100
| Spe1
| Pst1
| 887
|
|
| 17/07/07
|
| D3
| pJ23107
| pJ23107
| Spe1
| Pst1
| 2096
| Vector with the J23107 promoter ready for insertion of a RBS/CDS insert
|
| 17/07/07
|
| D4
| mRFP
| pJ23107
| Spe1
| Pst1
| 887
|
|
| 17/07/07
|
| D5
| pJ23100
| pJ23100
| EcoR1
| Spe1
| 2925
| The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.
|
| 17/07/07
|
| D6
| pJ23107
| pJ23107
| EcoR1
| Spe1
| 2925
| The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.
|
| 17/07/07
|
| D7
| Lox66 DapA E.coli
| Lox66 DapA E.coli (PCR product)
| EcoR1
| Pst1
| 977
|
|
| 17/07/07
|
| D8
| Lox66 DapA E.coli
| Lox66 DapA E.coli (PCR product)
| Xba1
| Pst1
| 962
|
|
| 17/07/07
|
| D9
| pJ23100 ES vector
| pJ23100 (MP1.1 & MP1.2)
| EcoI
| SpeI
| 2096
| Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert)
|
| 21/07/07
|
| D10
| pJ23100 promoter fw-insert
| pJ23100 (MP1.1 & MP1.2)
| EcoI
| SpeI
| 58
| J23100 promoter ready for use as a fw-insert
|
| 21/07/07
|
| D11
| pJ23107 ES vector
| pJ23107 (MP2.1, MP2.2, MP2.3 & MP2.4)
| EcoRI
| SpeI
| 2096
| Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter
|
| 21/07/07
|
| D12
| pJ23107 promoter fw-insert
| pJ23107 (MP2.1, MP2.2, MP2.3 & MP2.4)
| EcoRI
| SpeI
| 58
| J23107 promoter ready for usage as a fw-insert
|
| 21/07/07
|
| D13
| mRFP
| pJ23107 (MP2.2, MP2.3 & MP2.4)
| SpeI
| PstI
| 887
| mRFP RBS/CDS/T sequence, can it be used as a backward insert? => yes but you will not be able to add any biobrick after it
|
| 21/07/07
|
| D14
| Cre ORF
| J61047 MP9.1 & MP9.2
| XbaI
| PstI
| 1063
| Cre ORF extracted for usage as a bw-insert
|
| 21/07/07
|
| D15
| B0030 bw-vector
| B0030 MP5.1 & MP5.2
| SpeI
| PstI
| 2076
| bacward vector for cloning a CDS bw-insert after the B0030 RBS
|
| 21/07/07
|
| D16
| pSB1AK3 vector
| B0015 MP3.1 & MP3.2
| EcoRI
| PstI
| 3148
| pSB1AK3 open vector for BioBrick cloning
|
| 21/07/07
|
| D17
| pSB1A2 E0422 vector
| E0422 (MP6.1 & MP6.2)
| EcoRI
| PstI
| 2038
| pSB1A2 open vector for BioBrick cloning
|
| 21/07/07
|
| D18
| BB dig. lox66DapAE.coli
| lox66-DapAE.coli P5 PCR product
| EcoRI
| PstI
| ~960
| PCR product digested for cloning as a BioBrick
|
| 21/07/07
|
| D19
| BB dig. lox66DapAsubtilis
| lox66-DapAsubtilis P6 PCR product
| EcoRI
| PstI
| ~960
| PCR product digested for cloning as a BioBrick
|
| 21/07/07
|
| D20
| fw insert lox66-DapAE.coli
| lox66-DapAE.coli P5 PCR product
| XbaI
| PstI
| ~960
| PCR product digested for usage as a forward insert
|
| 21/07/07
|
| D21
| fw insert lox66-DapAsubtilis
| lox66-DapAsubtilis P6 PCR product
| XbaI
| PstI
| ~960
| PCR product digested for usage as a forward insert
|
| 21/07/07
|
| Strains glycerol stock
|
| Number
| Name
| Description
| Date
|
| S1
| MG1655
| Standard E.Coli strain
|
|
| S2
| Acinetobacter ADP1
| Strain used for TG coloration test with Red Nile
|
|
| S3
| DH5alpha pKs::DGAT
| Enzyme catalysing the transormation of DAG into TG
|
|
| S4
| BBa_pJ23100
| Strong constitutive promoter in J61002 (clones 1 + 2)
|
|
| S5
| BBa_B0015
| Double terminator (clones 1 + 2)
|
|
| S6
| BBa_pJ23107
| Medium constitutive promoter in J61002 (clones 1 + 2)
| 19/07/07
|
| S7
| BBa_B0030
| Strong RBS (clones 1 + 2)
| 19/07/07
|
| S8
| BBa_E0422
| ECFP (RBS+LVA+Term) (clones 1 + 2)
| 19/07/07
|
| S9
| BBa_E0241
| PoPs to GFP converter (clones 1 + 2)
| 19/07/07
|
| S10
| BBa_E0840
| gfp tri-part; strong rbs (clones 1 + 2)
| 19/07/07
|
| S11
| BBa_J61047
| Cre ORF (clones 1 + 2)
| 19/07/07
|
| S12
| W121
|
| 05/07/07
|
| S13
| BBa_I0500
| pBAD AraC (clone 1+2)
| 20/07/07
|
| S14
| BBa_pJ23107
| Medium constitutive promoter in J61002 (clone 3+4)
| 20/07/07
|
