Boston University/Plasmid Customization

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Overview

Excising the sacB gene from plasmid pJQ200

PCR amplification of lacZ

BU labeled digested pjq200 7-19-2007.jpg

BU labeled pjq200 sacb digestion 7-17.jpg

Verifying the presence of the lacZ promoter in the plasmid pJQ200

Double digestion of pJQ200 with EcoRI and HindIII

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