From 2007.igem.org

1. Autoclave LB medium with 2% agar.

2. Cool at 50 °C to prevent agar polymerization.

3. Before preparing plates add antibiotic (Ampicillin 1000X [ ] or Kanamicin 200X [ ]).

4. Put about 20 ml of medium per plate.

5. Leave it solidify and store at 4°C.

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