| Name
| Sequence
| Length
| Tm (C)
| Restriction site
| Target length
| Description
|
| M_INS_A 5'
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGGCATTGTAGATCAGTGCTG
| 51 bp
| 56.85
| EcoRI, XbaI
| 63 bp
| Potential dimer formation!
|
| M_INS_A 3'
| GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT
| 55 bp
| 54.74
| SpeI, PstI
| 63 bp
| Potential dimer formation!
|
| M_INS_B 5'
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTGT
| 52 bp
| 60.85
| EcoRI, XbaI
| 90 bp
|
|
| M_INS_B 3'
| GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA
| 55 bp
| 55.86
| SpeI, PstI
| 90 bp
|
|
| RcsC 5'
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAATACCTTGCTTCTTTTCG
| 51 bp
| 55.92
| EcoRI, XbaI
| 1396 bp
|
|
| RcsC 3'-2
| GGGGTATACCAACGACTCTTCCATCTTCAC
| 29 bp
| 59.63
| NdeI
| 1396 bp
| Two extra adenosine was added to the 5' side of the NdeI cutting site.
|
| [http://partsregistry.org/Part:BBa_G00100 VF2]
| tgccacctgacgtctaagaa
| 20 bp
| 59.44
|
|
| Forward sequencing primer.
|
| [http://partsregistry.org/Part:BBa_G00101 VR]
| attaccgcctttgagtgagc
| 20 bp
| 59.34
|
|
| Reverse sequencing primer.
|
}
- [http://www.mdbio.com.tw/pfu.htm pfu primer]
