NYMU Taipei/Primer List

Revision as of 09:10, 21 August 2007 by Lihsiangyen (Talk | contribs)

Name	Sequence	Length	Tm (C)	Restriction site	Target length	Description
M_INS_A 5'	GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGGCATTGTAGATCAGTGCTG	51 bp	56.85	EcoRI, XbaI	63 bp	Potential dimer formation!
M_INS_A 3'	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT	55 bp	54.74	SpeI, PstI	63 bp	Potential dimer formation!
M_INS_B 5'	GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTGT	52 bp	60.85	EcoRI, XbaI	156 bp	Potential dimer formation!
M_INS_B 3'	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA	55 bp	55.86	SpeI, PstI	156 bp	Potential dimer formation!
H_INS_A 5'	GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGGCATTGTGGAACAATGCTG	51 bp	62.45	EcoRI, XbaI	129 bp	Potential dimer formation!
H_INS_A 3'	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCTG	56 bp	57.77	SpeI, PstI	129 bp	Potential dimer formation!
H_INS_B 5'	GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTGAACCAACACCTGTG	51 bp	57.98	EcoRI, XbaI	156 bp	Potential dimer formation!
H_INS_B 3'	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGTCTTGGGTGTGTAGAAGA	55 bp	55.10	SpeI, PstI	156 bp
RcsC 5'	GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAATACCTTGCTTCTTTTCG	51 bp	55.92	EcoRI, XbaI	1404 bp
RcsC 3'-2	GGGGTATACCAACGACTCTTCCATCTTCAC	29 bp	59.63	NdeI	1404 bp	Three extra guanosine was added to the 5' side of the NdeI cutting site.
[http://partsregistry.org/Part:BBa_G00100 VF2]	tgccacctgacgtctaagaa	20 bp	59.44			Forward sequencing primer.
[http://partsregistry.org/Part:BBa_G00101 VR]	attaccgcctttgagtgagc	20 bp	59.34			Reverse sequencing primer.