NYMU Taipei/Lab Notes/2007 9 9

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Revision as of 10:30, 10 September 2007 by Lihsiangyen (Talk | contribs)
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  • Ligation(H_INS_A)
    • H_INS_A1:1ul
    • pOmpC:2ul
    • 10X ligase:1 ul
    • 10X buffer:1 ul
    • ddH2O:5ul
    • total: 10ul
  • ligation time:1hr30min
  • Transformation
    • ligated DNA:2ul
    • compenent cell:100ul
  • apply the cells on the agar plate
  • plate culture failed!
  • possibility:concentration of H_INS_A1 is not high enough
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