From 2007.igem.org

MENU : Introduction | Applications | Design | Modelling | Status | References

Project Description

The aim is to create a signal generator device that produces an output of PoPS as a function of bacterial cell division. Downstream of the device may be a counter device, quantifiable protein production or some other function of choice.

The system may for example perform pre-determined actions such as programmed cell death after a set number of cell divisions.

The device relies on dif recombinase sites to flip a sequence during each cell division. We hope to further analyse cell division and recombinase mechanisms since bacterial cell division is still relatively poorly understood.

We have constructed and are ligating the bricks required for the first proof of concept experiments.

The structure of the wiki for this project is this:

• This Introduction page with a brief overview.
• The Applications page with some possible uses of the Division PoPper.
• The Design page that explain what are the logic and functional elements in the construct.
• The Realization page that explain what are the biological elements we use to implement the construct (decoupled from design as the Synthetic Biology approach requires).
• The Modelling page containing the computational and mathematical investigation of the system behaviour.
• The Status page that reports what we have achieved so far.
• The References page that lists the background papers validating our work.

A menu with the same links is present at the top of each page for facilitating navigation.