Week 12
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- 09/17/07
- Ligations for:
-I763026 + I763019;
-I763026 + I763004;
-I763025 + J04031.
- We transform ligations and strake them on plates.
- 09/18/07
- 09/19/07
- Miniprep for:
-I763027;
-J04431;
-Ptet-LacI-GFP(Spe/Pst1), (Xba/Pst1).
- Digestion for:
-I763027 with Eco/Spe;
-J04431 with Eco/Spe;
-Ptet-LacI-GFP with Eco/Spe;
-I763007 with Eco/Xba;
-Plac-cI-LacY with Eco/Spe1;
- Band extraction from gel for all digestion and then we observe:
-I763027, J04431 are died;
-Ptet-LacI-GFP is correct;
-I763007 not found;
-Plac-cI-LacY is correct.
- 09/20/07
- 09/21/07
