Week 12
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Revision as of 13:52, 20 September 2007 by Silvia.tamarri2 (Talk | contribs)
- 09/17/07
- Ligations for:
-[http://partsregistry.org/Part:BBa_I763026 I763026] + -[http://partsregistry.org/Part:BBa_I763019 I763019];
-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763004 I763004];
-[http://partsregistry.org/Part:BBa_I763025 I763025] + [http://partsregistry.org/Part:BBa_J04031 J04031].
- We transform ligations and strake them on plates.
- 09/18/07
- We inoculate a colony for yesterday ligations in 5ml of LB medium O/N.
- 09/19/07
- Miniprep for:
-[http://partsregistry.org/Part:BBa_I763027 I763027];
-[http://partsregistry.org/Part:BBa_J04031 J04031]J04431;
-Ptet-LacI-GFP(Spe/Pst1), (Xba/Pst1).
- Digestion for:
-I763027 with Eco/Spe;
-J04431 with Eco/Spe;
-Ptet-LacI-GFP with Eco/Spe;
-I763007 with Eco/Xba;
-Plac-cI-LacY with Eco/Spe1;
- Band extraction from gel for all digestion and then we observe:
-I763027, J04431 are died;
-Ptet-LacI-GFP is correct;
-I763007 not found;
-Plac-cI-LacY is correct.
- 09/20/07
- 09/21/07
