From 2007.igem.org

<return to list of secondary methods> <team home page>

Contents 1 Preparation of LB 2 Uses 3 Ingredients: 4 Method:

Preparation of LB

Uses

• Culturing Bacteria
• Making LB-Agar Plates

Ingredients:

For 1L:

• 10g Bacto-tryptone
• 5g Yeast Extract
• 10g NaCl
• NaOH to adjust pH

Method:

1. Place Ingredients in a plastic container and add sterile water to the desired volume.
2. Mix using a magnetic stirrer and adjust pH using NaOH until the pH is 7.5.