From 2007.igem.org

1. Growth O/N for 15h in LB (5ml) of:

-trasformed E.Coli with appropriate antibiotic;

-E. Coli.

2. All misure has to be done in M9 at OD=1.

3. The day after in the morning OD measure.

4. To obtain OD=1:

-centrifugate 4ml of bacteria culture at 4400rpm for 3min at 25°C;

-eliminate the supernatant;

-risuspende the pellet with 6ml in M9 medium with glucose with appropriate antibiotic

       (1000x).

5. OD measure: adjust OD to 1 through further diluition or growth.

6. Adjust PMT offset with untrasformed E.Coli.

7. Fluorescence test of the culture before IPTG induction.

8. IPTG induction:

-centrifugate bacteria culture at 4400rpm for 3min at 25°C;

-eliminate the supernatant;

-risuspende the pellet in M9 medium and 2mM IPTG with appropiate antibiotic (1000X);

9. Incubate at 37°C.

10. Fluorescence test after10min, 20min, 30min, 1h, 2h.