From 2007.igem.org

Vaibhaviu 19:02, 1 June 2007 (EDT)

Today we cloned an RFP basic part into pBca9145. So, we did the construction file for pBca9145-Bca1145. We did a PCR of the RFP gene according to the construction file, and the gel is here:

It's a little dilute, but it should work nonetheless. We also digested the vector according to the construction file:

We gel purified the larger of the two bands. It looks like the digest went pretty well, as we see no single-cut plasmid DNA. We took it all the way through to transformation into DH10B cells today.