From 2007.igem.org

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Contents 1 Oct. 4, 2007 2 Oct. 3, 2007 3 Oct. 2, 2007 4 Oct. 1, 2007

Oct. 4, 2007

Esther, Neha, Rafsan

SUMMARY:

• Found alternate to I1303: ligate terminator (B0015) and Lux pR promoter (R0062) separately
• Transformed R0062 (2007), B0015 (2005)
• Made o/n of DH5a, DH5a-z1, J06801 (2005), Q04400 (2005), B0034 (2007), J31005 (2007)
• Digest and Gel Extract of N1 A with enzymes S/P
• Quantitation: N1 = ng/uL, S01003 (2007) = ng/uL

TO DO:

• Make o/n: R0062 (2007), B0015 (2005)
• Miniprep and Check Lengths: J06801 (2005), Q04400 (2005), B0034 (2007), J31005 (2007)
• Ligate N1 and S01003 and transform in DH5a
• Make competent cells

Oct. 3, 2007

Yusuf

SUMMARY:

• Transformed: J06801 (2005), Q04400 (2005), B0034 (2007), J31005 (2007)
• Minipreped I130033 AB, E0433 AB, S03140 AB, J04500 AB, J31003 AB, N1 AB
• Made o/n of DH5a and DH5a-z1 (from July 16 plate - ask Seema if this is ok)
• Length Check (O = match, X = does not match):

Part Name	Part	Plasmid
E0433 A	O	O
E0433 B	O	O
I13033 A	X	O
I13033 B	X	O
J04500 A	O	O
J04500 B	O	O
J31003 A	O	O
J31003 B	O	O
N1 A	O	O
N1 B	O	O
S03140 A	O	O
S03140 B	O	O

TO DO:

• Make 5 Kan plates
• Make bottle of LB Broth
• Make competent cells
• Make o/n of J06801 (2005), Q04400 (2005), B0034 (2007), J31005 (2007)
• Ligate N1 + S01003
• Find alternate to I13033

Oct. 2, 2007

Natalie

SUMMARY:

• Made o/n (2 each) of: I130033 AB, E0433 AB, S03140 AB, J04500 AB, J31003 AB, N1 AB
• NOTE: N1 = J34100 C + S01640 B + B0015

TO DO:

• Miniprep all above parts and length check
• Transform:
  • J06801 (2005) - it's in the source box (this is a larger composite part containing J06501)
  • B0034 - also in source box, if not, get it from Registry plates
  • Q04400 - re-transform using more DNA to see if we get more colonies and transform 2005/2006 version in the source box
  • J31005 - chloramphenicol resistance, just in case we need it
• Continue cataloging and organization (Charles: I'll be dropping by to help out with that)

Oct. 1, 2007

Time: 3:45 PM - 8:30 PM

Yusuf, Irina

Summary:

• made 5 KAN plates
• made 2 AMP+KAN plates
• made 300 mL LB

Tranformation of following parts:

• I13033 -> KAN
• E0433 -> KAN
• S03140 -> AMP
• J04500 -> KAN
• J31003 -> AMP
• (J23100C + S01640B)+ B0015 -> KAN

Used 4uL of DNA for all transformations

• tried to do DNA extraction for J06501 but somebody already extracted it (wasn't in the well)
• couldn't find it in the igem dna box as well !!!

TO DO:

• MP o/n for the above parts