From 2007.igem.org

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Contents 1 Week 5 2 Week 6 3 Week 7 4 Week 8

Week 5

23 July 2007

• Colony PCRed using vf2 and vR primers the transformations from 20/7. 3 colonies from ligation plate and one from plasmid control plate.

24 July 2007

• Ran Gel from PCRs (23/7) at 100V.

-results: colony 3 didn't give product.

• Streaked out some cells from index plate (No.3).

25 July 2007

26 July 2007

27 July 2007

Week 6

30 July 2007

31 July 2007

• Diagnostic Digest of
  1. P2 21B
  2. P2 23N
  3. P3 20I

using miniprep from 13/7. All digests were E/P.

Ran a Gel:

• 1. DNA ladder
  2. P2 21B
  3. P2 21B
  4. P2 23N
  5. P2 23N
  6. P3 20I
  7. P3 20I

-Results:

• 2. Low digestion efficiency
  3. Low digestion efficiency
  4. No insert seen but DNA present
  5. No insert seen but DNA present
  6. Ok digestion (not very good)
  7. Ok digestion (not very good)

--Digestion imcomplete??

1 Aug 2007

• Transformation of P2 9E (LacZa with KanR) from iGEM plate.

2 Aug 2007

3 Aug 2007

4 Aug 2007

Week 7

5 Aug 2007

6 Aug 2007

7 Aug 2007

8 Aug 2007

9 Aug 2007

10 Aug 2007

11 Aug 2007

Week 8

12 Aug 2007

13 Aug 2007

14 Aug 2007

15 Aug 2007

16 Aug 2007

17 Aug 2007

18 Aug 2007