pBca1101-Bca1128 [RBS library pool, including pBca1101-Bca1106] (EcoR1/Bgl2, 2942/58/9, Largest) Bca1121 [Kan] (EcoR1/Bgl2, 2987/895, Smaller) Ligated product is pBca1101-Bad0001 (Now known as I716051)