BerkiGEM2007-SamanthaConstructionFile6
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Revision as of 22:56, 4 June 2007 by Samanthaliang (Talk | contribs)
Biobricking of barnase active codon
PCR sil03F and sil03R on barnase region (348 bp, BglII/XhoI)
Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L)
Product is pBca9145-I716210
sil03F Forward BglII biobrick of barnase ccaaaAGATCTgcacaggttatcaacacgtttg
sil03R Reverse XholI/BamHI biobrick of barnase gctagCTCGAGttaGGATCCttatctgatttttgtaaagg
