BerkiGEM2007-DavidConstructionFile103

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Revision as of 06:35, 5 June 2007 by Dtulga (Talk | contribs)
Biobrickificreation of T7rnap I716103, I716104 (T7 RNA Polymerase: ATG, GTG)
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PCR T7rnap-FA and T7rnap-R on pBAC-T7621	(2683 bp, BglII/XhoI, Product = A)
PCR T7rnap-FG and T7rnap-R on pBAC-T7621	(2683 bp, BglII/XhoI, Product = G)
Sub both into pBca9145-Bca1144			(BglII/XhoI, 2063+910, L)
Product A is pBca9145-I716103-T7rnap-A
Product G is pBca9145-I716104-T7rnap-G
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dt005-T7rnap-FA  Forward Cloning of T7rnap ATG   ccaaaAGATCTatgaacacgattaacatcgc
dt006-T7rnap-FG  Forward Cloning of T7rnap GTG   ccaaaAGATCTgtgaacacgattaacatcgc
dt007-T7rnap-R   Reverse Cloning of T7rnap       gctagCTCGAGttaGGATCCttacgcgaacgcgaagtccg
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