From 2007.igem.org

My Construction Files
My Sequencing Files

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6/6 waiting for oligos

• Made oligos and constructs with Vai, for getting wbbL and neuS from pJ23006-Bca9106

6/5 coolbeans

• Finalized oligos to order with Vai
• Learned about LB broth-ing and LB/Agar plating. Thanks, Austin and Sam :)
• Learned about the many composite part-making methods. Props 2 Chris

1. prefix/suffix is weaksauce
2. Use the AlwnI or BsaI or BglI, in conjuction with BglII or BamHI << (Did this today)
3. DBBS
4. 3 antibiotic; MIT endorses, used for BioBrick 1.0. Triple digest = bad
5. 1-2-3 method << 'Our Goal' in a few weeks. should be leet.

• Planned and vicariously did the making of P_tet+RFP brick (see Vai Notebook)

Other Notes: All oligos are being ordered, w00t w00t. Ayu 18:36, 5 June 2007 (EDT)

6/4 Training Finishes, Real Stuff Starts

• Incubated some colonies
• Miniprep'd already-been-incubated colonies (2)
• Double digest of the 2 minipreps + parent plasmid
• Colony PCR'd the incubated E.coli
• Ran gel of the digest + PCR

• >>> PCR product / Miniprep 1 / Parent Plasmid / Miniprep 2 / Ladder >>>
• No bands for PCR or parent. Confused? Other ones look great.

As for me: Wiki acc works now.
Designing oligos and will compare with Vai. Ayu 18:19, 4 June 2007 (EDT)

to do