BerkiGEM2007-KristinConstructionFile3

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Construction of hemC basic part
PCR kd007/kd0010 on hemC	(306 bp, gp=A)
PCR kd009/kd008 on hemC	        (688 bp, gp=B)
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PCR kd007/kd008 on A+B 	        (994 bp, Bg1II/XhoI)
Digest pBca9145-Bca1144#5  	(BglII/XhoI, 2063+910, L)
Product is pBca9145-I716154
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kd007	Forward Bg1II for hemC	     	ccaaaAGATCTatgataatgacggtaacaagc
kd008	Reverse Xho1 for hemC	     	gctagCTCGAGttaGGATCCtcatgccggggcgtctccgttatag
kd009	Forward remove the EcoR1 site in hemC       gccggttGAGTTCccgcaag
kd010	Reverse remove the EcoR1 site in hemC        cttgcggGAACTCaaccggc
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