Alberta/Calender/October
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== October 1 ==
JG
Miniprep J61003+Enny and Benny+J61003
Minis are in -20
ML
Brought the tubes labelled "sequencing rxns" up to MBSU
Also brought some XBA 1 from fermentas freezer since we ran out
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min
Colony O/N of I0500/ J61003 and Buddy/J61003
October 2
VH-1PM
No Kan plates therefore made kan plates
On COuntertop
Miniprepped ML's overnights from OCt 1
Lysis solution is a no go
Started new O/N of previows overnights for tomorrow
No more LB
== October 3 ==
MC - 800hrs
Autoclaved 2 bottosl of Ependorf tupes- to be picked up from G308
Transform THolase into XL10 gold plates
Miniprep of 10500+J61003 O/N
ML
Digest of 10500+J61003 with ECORI and XBA
Housekeeping complete
Note to Justin: Samples to sequence are in -20 labelled "Justin! Sequence me"
CZ - 7:00pm
Ran gel of I0500/J61003
It looks like I05oo is in J61003 but have to confirm with Justin or Michelle or Erin.
NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs
October 4
NK - 930
VH - 2pm
Cleaned up 37 degree
Moved 30th plates of I0500+JG and Buddy+j6 to 4 degree fridge
Disposed of really disgusting plates
Got ride of I0500 ovrnights that hve been on shaker for a wek now
CHecke thoolase kan plates
Showing blue dots so far and af we white doets
Left plates in 37 celsious room
JP
Sequencing of 3 primer of DBS
Ligate I0500 eco/spe into J61003 (eco/xba)
October 5
JG/MC - 800hrs
Ran gel of i0500 and buddy
Digest #4 J61003/I0500 Digest Eco/xba with Pst to drop out of GFP
Ran gel of I0500J61003 eco/xba
ML
Gel completd and took photos
Buddy and I0500 digested
Extracted gel
Labelled I0500 purify Oct5 and Buddy Purify oct 5
CZ - Sorry I can't make it for personal reasons.
October 6
ED 9:00
Made a to do list for the day
NK 2pm
Ligations of I0500 purify oct 5 and J61003
Left on bench with green tape labelled "ligations oct6"
Digest Boo34 with s,p
Digests in freezer labelled "digestions B0034 S,P"
October 7
ED 9:00
NG 12:00
October 8
October 9
NK
Loaded gel with digets from october 6, boo34 S,P
Put the ligations from oct6 in the freezer with the tape in tray #3
VH
Gel extrations B0034 sp1 and boo34 sp2
October 10
JP?
Tholase PCR
MC
Ligated Buddy oct 5 into Boo34 digest oct 6
Left on bench
October 11
JP?
PCR thiolase
Transformed Buddy in Boo 1+2 into competentent cells, plated on AMP+ plates
October 12
MC, JG @ 800hrs
Ran gel of PCR products
No growth on the buddy in Boo transformation
Religations of buddy into boo but couldnt find Buddy therefore religations halted
October 13
October 14
October 15
October 16
October 17
October 18
October 19
October 20
October 21
October 22
October 23
October 24
October 25
October 26
October 27
October 28
October 29
October 30
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