From 2007.igem.org

• Home
  • Site Map
  • Meet the Team
  • Fun with iGEM
  • IcGEMs@OpenWetware
  • Acknowledgements
• Infector Detector
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • F2620 Comparison
  • Conclusion
• Cell by Date
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • Conclusion
• Cell-Free
  • What is Cell-Free?
  • Cell-Free Vs. Cell
  • Our Contributions
  • Characterisation
  • Applications
• Wet Lab
  • Lab Notebook
  • Protocols & Results
  • DNA Constructs
• Dry Lab
  • Modelling
  • Data Analysis
  • Software

Our Design

To get around the problem of having bacteria in contact with food or medical devices in the applications of Infector Detector and Cell by Date respectively, we aimed to express reporter DNA constructs within a CFS consisting of cell extract encapsulated in selectively permeable vesicles. Our plan was to first prepare the cell extract and perform in vitro expression in bulk solution. In parallel, we would investigate and optimize conditions for phospholipid vesicle formation. The final stage of our project would involve combining the two, so that protein expression is achieved inside the vesicles.

Cell-free in Infector Detector

(Specs/Design based)

Cell-free in Cell by Date

(Specs/Design based)